Journal
BIOCHEMISTRY
Volume 49, Issue 8, Pages 1557-1559Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi100013s
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Funding
- National Science Foundation [CHE-0848398]
- University of Maryland
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0848398] Funding Source: National Science Foundation
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1200160] Funding Source: National Science Foundation
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Fluorinated analogues of tyrosine call be used to Manipulate the electronic environments of protein active sites. The ability to selectively Mutate tyrosine residues to fluorotyrosines is limited, however, and call Currently only be achieved through the total synthesis of proteins. As it general Solution to this problem, we genetically encoded the unnatural amino acids o-nitrobenzyl-2-fluorotyrosine, -3-fluorotyrosine, and -2,6-difluorotyrosine in Escherichia coli. These amino acids are disguised from recognition by the endogenous protein biosynthetic machinery, effectively preventing global incorporation of fluorotyrosine into proteins.
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