Journal
BIOCHEMISTRY
Volume 48, Issue 8, Pages 1785-1792Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi800708b
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- Deutsche Forschungsgemeinschaft [1150]
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Clostridium sordellii lethal toxin (TcsL) belongs to the family of clostridial glucosylating toxins. TcsL exhibits glucosyltransferase activity to inactivate Rho and Ras proteins. On Cultured cells, TcsL causes actin reorganization (cytopathic effect) and apoptotic cell death (cytotoxic effect). This study is based on the concept that the cytotoxic effects of TcsL depend on the glucosylation of critical substrate proteins rather than on the glucosyltransferase activity per se. The cytotoxic effects of TcsL depend on the glucosyltransferase activity of TcsL, as neither chemically inactivated TcsL nor a glucosyltransferase-deficient mutant version of TcsL caused it. The TcsL homologous toxin B from Clostridium difficile serotype F strain 1470 (TcdBF) also failed to cause cytotoxic effects. Correlation of the toxins' respective protein substrate specificities highlighted (H/K/N)Ras as critical substrate proteins for the cytotoxic effects. (H/K/N)Ras are critical upstream regulators of phosphatidylinositide 3'-OH kinase (P13K)/Akt survival signaling. Tauroursodeoxycholic acid (TUDCA) classified to activate P13K/Akt signaling downstream of apoptosis-inducing stimuli prevented the cytotoxic effects of TcsL. In conclusion, (H/K/N)Ras glucosylation and subsequent inhibition of P13K/Akt signaling are critical for the cytotoxic effects of TcsL.
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