Journal
BIOCHEMISTRY
Volume 48, Issue 44, Pages 10483-10485Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi901519q
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Funding
- Boston University
- NSF CAREER [CHE-0748504]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0748504] Funding Source: National Science Foundation
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IspG is a [4Fe-4S] cluster-containing protein, and the [4Fe-4S](+) species is proposed to be the catalytically relevant species. However, attempts reported in the literature failed to detect the [4Fe-4S](+) species In this study, using a potent reduction system, we have successfully detected the [4Fe-4S](+) species with X-band EPR spectroscopy. In addition, we have improved the Escherichia coli IspG activity to 550 nmol min(-1) mg(-1), which is similar to 20-fold greater than that of the NADPH-Fpr-FldA system in the literature.
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