Characterization of lymphocyte subsets from mucosal tissues in neonatal swine

Monitoring differences in lymphocytes during neonatal development constitutes a key to understanding the developing piglet's natural and pathological immune responses. A survey was conducted to accumulate information on the phenotype of lymphocytes isolated from blood, lymph nodes, and lymphoid associated structures of the pig small intestine of conventional pigs from day 1 to 47 of age and inbred miniature pigs between 12 and 82 days. The effect of weaning, and age before and after weaning, were also evaluated. Weaning had a significant effect on the number of CD4(+): CD8(+), double positive CD4(+)/CD8(+), CD21(+), delta gamma TCR+, SWC3(+) and SLA-DQ(+) cells. Aging of the pig before and after weaning resulted in significant changes in lymphocytes isolated from mesenteric lymph nodes and ileal sites. These results constitute an important baseline for studying mucosal immune response of neonatal pigs and identifying factors that influence the ability of the neonate to respond to the stresses and antigenic exposure associated with weaning. Published by Elsevier Science Ltd.