Journal
JOURNAL OF MASS SPECTROMETRY
Volume 36, Issue 4, Pages 355-369Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/jms.153
Keywords
mass spectrometry; imaging; tissue; secondary ion mass spectrometry; matrix-assisted laser desorption/ionization
Funding
- NIGMS NIH HHS [GM 58008, GM 41617] Funding Source: Medline
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Organic secondary ion mass spectrometry (SIMS) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry can be used to produce molecular images of samples. This is achieved through ionization from a clearly identified point on a flat sample, and performing a raster of the sample by moving the point of ionization over the sample surface. The unique analytical capabilities of mass spectrometry for mapping a variety of biological samples at the tissue level are discussed. SIMS provides information on the spatial distribution of the elements and low molecular mass compounds as well as molecular structures on these compounds, while MALDI yields spatial information about higher molecular mass compounds, including their distributions in tissues at very low levels, as well as information on the molecular structures of these compounds. Application of these methods to analytical problems requires appropriate instrumentation, sample preparation methodology, and a data presentation usually in a three-coordinate plot where x and y are physical dimensions of the sample and z is the signal amplitude. The use of imaging mass spectrometry is illustrated with several biological systems. Copyright (C) 2001 John Wiley & Sons, Ltd.
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