Leucine 85 Is an Important Determinant of Divalent Ion Affinity in Rat β-Parvalbumin (Oncomodulin)

Despite 69% sequence identity with chicken parvalbumin 3 (CPV3), rat beta-parvalbumin (beta-PV) exhibits a substantially lower Ca(2+) affinity (Delta Delta G(o)' = 2.0 kcal/mol). This difference largely reflects the disparate behavior of the respective CD sites. Replacement of the rat beta-PV codon with the CPV3 codon at positions 49, 50, and 57-60 produces virtual sequence identity with the CPV3 CD site. However, the resulting protein exhibits a modest (0.5 kcal/mol) improvement in Ca(2+) affinity, implying that sequence differences beyond the binding site modulate divalent ion binding behavior. The solution structure of Ca(2+)-free rat beta-PV suggested that Leu-85, phenylalanine in CPV3, might be an important determinant. Therefore, the impact of the L85F mutation on divalent ion affinity was examined in rat beta-PV, in the variant harboring all six of the aforementioned CD site mutations, and in the intermediate CD site variants. We find that the identity of residue 85, located within the E helix, strongly influences divalent ion affinity in the mammalian beta-PV isoform and that its impact is mediated by interactions with residues in the CD site. In the wild-type protein, L85F primarily affects the EF site. By contrast, in the presence of the six CD site mutations, L85F also improves the CD site performance, yielding a protein with Ca(2+) affinity rivaling that of CPV3 and markedly enhanced Mg(2+) affinity as well. The impact of L85F on CD site Ca(2+) affinity is particularly sensitive to the identities of residues 59 and 60. Interestingly, however, significant improvement in CD site Mg(2+) affinity also requires mutation of additional CD site residues.