Journal
BIOCHEMISTRY
Volume 47, Issue 7, Pages 2190-2198Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi7021552
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- NIAID NIH HHS [R37 AI030557] Funding Source: Medline
- NIGMS NIH HHS [P01 GM072694] Funding Source: Medline
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We showed previously that cholesterol-rich liquid-ordered domains with lipid compositions typically found in the outer leaflet of plasma membranes induce liquid-ordered domains in adjacent regions of asymmetric lipid bilayers with apposed leaflets composed of typical inner leaflet lipid mixtures [Kiessling, V., Crane, J. M., and Tamm, L. K. (2006) Biophys. J. 91, 3313-26]. To further examine the nature of transbilayer couplings in asymmetric cholesterol-rich lipid bilayers, the effects on the lipid phase behavior in asymmetric bilayers of different lipid compositions were investigated. We established systems containing several combinations of natural extracted and synthetic lipids that exhibited coexisting liquid-ordered (l(o)) and liquid-disordered (l(d)) domains in a supported bilayer format. We find that to phase domains are induced in all quaternary inner leaflet combinations composed of PCs, PEs, PSs, and cholesterol. Ternary mixtures of PCs/PEs/Chol, PCs/PSs/Chol also exhibit l(o) phases adjacent to outer leaflet to phases. However, with the exception of brain PC extracts, binary PC/Chol mixtures are not induced to form to phases by adjacent outer leaflet to phases. Higher melting lipid ad-mixtures of PEs and PSs are needed for to phase induction in the inner leaflet. It appears that the phase behavior of the inner leaflet mixtures is dominated by the intrinsic chain melting temperatures of the lipid components, rather than by their specific headgroup classes. In addition, similar studies with synthetic, completely saturated lipids and cholesterol show that lipid oxidation is not a factor in the observed phase behavior.
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