Journal
BIOCHEMISTRY
Volume 47, Issue 45, Pages 11771-11782Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi801425e
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Funding
- European Commission Sixth Framework Programme COSBICS [LSHG-CT-2004-512060]
- Deutsche Forschungsgemeinschaft [DFG/Ke904]
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The formation of signal-promoting dimeric or oligomeric receptor complexes at the cell surface is modulated by self-interaction of their transmembrane (TM) domains. To address the importance of TM domain packing density for receptor functionality, we examined a set of asparagine mutants in the TM domain of the erythropoietin receptor (EpoR). We identified EpoR-T242N as a receptor variant that is present at the cell surface similar to wild-type EpoR but lacks visible localization in vesicle-like structures and is impaired in efficient activation of specific signaling cascades. Analysis by a molecular modeling approach indicated an increased interhelical distance for the EpoR-T242N TM dimer. By employing the model, we designed additional mutants with increased or decreased packing volume and confirmed a correlation between packing volume and biological responsiveness. These results propose that the packing density of the TM domain provides a novel layer for fine-tuned regulation of signal transduction and cellular decisions.
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