Journal
FISHERIES SCIENCE
Volume 67, Issue 2, Pages 333-340Publisher
SPRINGER JAPAN KK
DOI: 10.1046/j.1444-2906.2001.00233.x
Keywords
apoptosis; caspase; Danio rerio; embryo; heat shook; UV; zebrafish; gamma-ray irradiation
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Environmental stress-induced apoptosis in zebrafish Danio rerio embryos was characterized by assaying caspase-3-like activity and whole-mount terminal deoxynucleotidyl nick-end labeling (TUNEL) staining. Severe stress conditions, such as heat shock at 39 degreesC for 1 h, ultraviolet light at 10-100 mJ/cm(2) and gamma -ray irradiation at 5-20 Gy induced extensive apoptosis in embryos. Apoptotic cells were observed after the bud and 1-somite stages in normal embryos by TUNEL staining, and after stress treatment many TUNEL-positive cells were found in the enveloping and deep cell layers and the larval fin. The caspase-3-like activity increased severalfold during stress-induced apoptosis in a dose-dependent manner. These findings indicate that apoptotic pathways, mediated by caspase-3-like activity, play a major role in zebrafish embryogenesis under stress conditions.
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