Journal
FERTILITY AND STERILITY
Volume 75, Issue 4, Pages 674-677Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0015-0282(00)01796-9
Keywords
sperm; DNA denaturation; DNA fragmentation; sperm motility; infertility
Categories
Ask authors/readers for more resources
Objective: To evaluate two different assays of human sperm DNA integrity, DNA denaturation (DD) and DNA fragmentation (DF), and to correlate these with standard semen parameters. Design: Prospective, observational study. Setting: University infertility clinic. Patient(s): Forty consecutive semen samples from 33 nonazoospermic men presenting for infertility evaluation and 7 fertile men presenting for vasectomy. Intervention(s): Assessment of sperm concentration, motility, morphology, DD and DF. Main Outcome Measure(s): Sperm DD and DF in fertile and infertile men. Results: The mean (+/- SE) rates of DD and DF were significantly higher in infertile subjects compared to fertile controls, respectively: 25.4 +/- 3.0 vs. 10.2 +/- 2.3 (P=.028) and 27.6 +/- 2.5 vs. 13.3 +/- 2.5% (P=.016). DF and DD correlated strongly (r = 0.71, P<.0001). Also, DD and DF correlated negatively with standard semen parameters (concentration, motility, and morphology), the Strongest correlation being with sperm motility. Conclusion(s): The strong correlation between sperm DD and DF, and the higher levels of sperm DNA damage in infertile compared with fertile men. indicate that male infertility is associated with poor sperm DNA integrity. Although infertile men may father children with assisted conception, fertilization with DNA-damaged spermatozoa may increase the risk of genetic disease in the offspring. <(c)> 2001 by American Society for Reproductive Medicine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available