Journal
JOURNAL OF CHROMATOGRAPHY B
Volume 753, Issue 2, Pages 413-419Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00553-3
Keywords
oxalic acid; dopamine; serotonin
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Use of antioxidative agents is required in automated LC assay of microdialysis samples, due to rapid degradation of the monoamine neurotransmitters and their metabolites. Addition of oxalic acid prevented degradation of dopamine, serotonin, 3,4-dihydroxypheaylacetic acid, homovanillic acid and 5-hydroxyindoleacetic acid efficiently: after a 24-h incubation at room temperature the decreases in peak heights were less than 10%. The long-term stability of the analytes, however, was still enhanced when acetic acid and L.-cysteine were included in the solution. Using this antioxidative solution, the monoamine neurotransmitters and their. metabolites could be determined with an automated LC assay even at room temperature. (C) 2001 Elsevier Science B.V. All rights reserved.
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