Journal
SCIENCE
Volume 292, Issue 5514, Pages 110-113Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1060118
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- NIGMS NIH HHS [GM53512, GM59772] Funding Source: Medline
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The assembly of higher order chromatin structures has been Linked to the covalent modifications of histone tails. We provide in vivo evidence that lysine 9 of histone H3 (H3 Lys(9)) is preferentially methylated by the Clr4 protein at heterochromatin-associated regions in fission yeast. Both the conserved chromo- and SET domains of Clr4 are required for H3 Lys(9) methylation in vivo. Localization of Swi6, a homolog of Drosophila HP1, to heterochomatic regions is dependent on H3 Lysg methylation. Moreover, an H3-specific deacetylase Clr3 and a beta -propeller domain protein Rik1 are required for H3 Lys(9) methylation by Clr4 and Swi6 Localization. These data define a conserved pathway wherein sequential histone modifications establish a histone code essential for the epigenetic inheritance of heterochromatin assembly.
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