Journal
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
Volume 50, Issue -, Pages 139-146Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bse.2013.03.004
Keywords
Garlic germplasms; SRAP; Genetic diversity; Clustering analysis; Principal component analysis
Funding
- Yulin City Science and Technology Bureau project
- Tang Zhong-ying Breeding Foundation of Northwest AF University [A212020911]
- National Public Welfare Research Project [200903018-7]
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Cluster analysis and principal component analysis were used to investigate the genetic diversity of 40 garlic germplasms analyzed with 23 sequence-related amplified polymorphism (SRAP) primer combinations. A total of 130 polymorphic loci were detected among these germplasms, with an average of 5.65 polymorphic loci per SRAP primer combination. The percentage of polymorphic loci was 69.1%, whereas the mean effective number of alleles, the mean Nei's gene diversity, and the mean Shannon's information index were 1.4446, 0.2788, and 0.4365, respectively. Cluster analysis revealed that the 40 germplasms could be divided into 3 groups. The results of principal component analysis were consistent with those of unweighted pair-group method with arithmetic averages (UPGMA) clustering analysis. The Shannon-Weaver information index ranged from 0.2419 to 0.4202, indicating that the garlic germplasms had high genetic diversity. (C) 2013 Elsevier Ltd. All rights reserved.
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