Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 913, Issue 1-2, Pages 309-313Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(00)01201-2
Keywords
amino acids; homocysteine; cysteine; glutamic acid
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A method for the simultaneous measurement of two biologically important thiol compounds cysteine and homocysteine and five amino acids including neurotransmitters aspartate and glutamate is reported. This method utilized derivatization of compounds with o-phthalaldehyde in the presence of 2-mercaptoethanol following alkylation of the free sulfydryl group with iodoacetic acid followed by separation using reversed-phase high-performance liquid chromatography. These o-phthalaldehyde -2-mercaptoethanol-labeled compounds were separated within 30 min on a Spherisorb ODS-2 column with isocratic lution using 17% methanol, 0.04 M sodium phosphate buffer (pH 7.0), 0.002 M Na(2)EDTA and detected fluorimetrically (excitatian 340 nm, emission 450 nm). Using this method, the concentrations of homocysteine, cysteine, glutamic acid, aspartic acid, asparagine, serine and glutamine in human plasma were determined. (C) 2001 Elsevier Science B.V. All rights reserved.
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