Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 913, Issue 1-2, Pages 283-302Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(00)01200-0
Keywords
reviews; derivatization; LC; amino acids; amino acids; OPA derivatives
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An overview is presented of HPLC methods currently in use to determine amino acids as their o-phthaldialdyde derivatives in the presence of various SH-group-containing additives. Crucial points that proved to influence the stability of the amino acid OPA derivatives have been discussed in detail: (i) the mol ratios of the OPA-SH-group-containing additive amino acid; (ii) the preparation and storage conditions of the OPA reagents; (iii) the optimum pH conditions for the interactions and elutions; (iv) the behavior of the, believed to be, less stable amino acids, such as glycine, beta -alanine, gamma -aminobutyric acid, histidine, ornithine and lysine. (C) 2001 Elsevier Science B.V. All rights reserved.
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