Journal
FEMS MICROBIOLOGY LETTERS
Volume 197, Issue 2, Pages 179-186Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2001.tb10601.x
Keywords
FTIR spectroscopy; cell wall structure; alpha-glucosidase; beta-glucan; mannoprotein; Saccharomyces cerevisiae
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A new strain, exhibiting an intriguing pink-colored cell phenotype was obtained after an encoding alpha -glucosidase gene from an archaebacteria Thermococcus hydrothermalis was cloned by Functional complementation of a mul11 Saccharomyces cerevisiae mutant TCY70. The possible implications of the alpha -glucosidase on the cell wall were evaluated by infrared spectroscopy and data indicate a 30% decrease in mannoproteins and an increase in beta -glucans. The loss of mannoproteins was confirmed by experiments on cells deprived of peptidomannans. Modifications in the major components of the cell wall did not jeopardize cell viability. Such rapid optical spectroscopic method can be used to screen a wide range of yeast mutants. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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