Journal
JOURNAL OF NEUROSCIENCE
Volume 21, Issue 8, Pages 2589-2599Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.21-08-02589.2001
Keywords
ligand-gated ion channel; glycine receptor alpha 1 subunit; substituted cysteine accessibility method (SCAM); methanethiosulfonate ethyltrimethlammonium (MTSET); methanethiosulfonate ethylsulfonate (MTSES); hyperekplexia
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Mutations in the extracellular M2-M3 loop of the glycine receptor (GlyR) alpha1 subunit have been shown previously to affect channel gating. In this study, the substituted cysteine accessibility method was used to investigate whether a structural rearrangement of the M2-M3 loop accompanies GlyR activation. All residues from R271C to V277C were covalently modified by both positively charged methanethiosulfonate ethyltrimethylammonium (MTSET) and negatively charged methanethiosulfonate ethylsulfonate (MTSES), implying that these residues form an irregular surface loop. The MTSET modification rate of all residues from R271C to K276C was faster in the glycine-bound state than in the unliganded state. MTSES modification of A272C, L274C, and V277C was also faster in the glycine-bound state. These results demonstrate that the surface accessibility of the M2-M3 loop is increased as the channel transitions from the closed to the open state, implying that either the loop itself or an overlying domain moves during channel activation.
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