Journal
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
Volume 38, Issue 5, Pages 981-987Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bse.2010.09.006
Keywords
Antheraea pernyi; Myosin light chain 2; Gene cloning; Expression pattern; Phylogeny
Funding
- National Modern Agriculture Industry Technology System Construction Project
- Scientific Research Project for High School of the Educational Department of Liaoning Province [2008643]
- Shenyang Agricultural University [2009023]
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Myosin light chain 2 (MLC-2) gene was isolated and characterized from Antheraea pernyi, a well-known wild silkmoth. The isolated cDNA sequence is 905 bp in length with an open reading frame of 612 bp encoding a polypeptide of 203 amino acids. Semi-quantitative RT-PCR analysis showed that the MLC-2 gene was transcribed during four developmental stages (egg, larva, pupa, and moth), and present in all tissues tested. Alignment analysis revealed that the deduced protein sequence has over 95% identity to myosin light chain 2 of lepidopteran species, and 57-88% identity to other insect species, suggesting that insect MLC-2 proteins are highly conserved throughout evolution. The protein sequence was used to construct phylogenetic trees with other known vertebrate and invertebrate MLC-2 sequences, and the obtained trees demonstrated similar topology with the classical systematics, indicating the potential value of MLC-2 gene in phylogenetic, study. (C) 2010 Elsevier Ltd. All rights reserved.
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