Journal
EMBO JOURNAL
Volume 20, Issue 8, Pages 1829-1839Publisher
WILEY
DOI: 10.1093/emboj/20.8.1829
Keywords
antibiotics; edeine; IF3; ribosomes; tetracycline
Categories
Funding
- NIGMS NIH HHS [GM34360, R01 GM034360] Funding Source: Medline
Ask authors/readers for more resources
The small ribosomal subunit is responsible for the decoding of genetic information and plays a keg role in the initiation of protein synthesis. We analyzed by X-ray crystallography the structures of three different complexes of the small ribosomal subunit of Thermus thermophilus with the A-site inhibitor tetracycline, the universal initiation inhibitor edeine and the C-terminal domain of the translation initiation factor IF3, The crystal structure analysis of the complex with tetracycline revealed the functionally important site responsible for the blockage of the A-site, Five additional tetracycline sites resolve most of the controversial biochemical data on the location of tetracycline, The interaction of edeine with the small subunit indicates its role in inhibiting initiation and shows its involvement with P-site tRNA, The location of the C-terminal domain of IF3, at the solvent side of the platform, sheds light on the formation of the initiation complex, and implies that the anti-association activity of IF3 is due to its influence on the conformational dynamics of the small ribosomal subunit.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available