Journal
FEBS LETTERS
Volume 495, Issue 1-2, Pages 87-93Publisher
WILEY
DOI: 10.1016/S0014-5793(01)02358-4
Keywords
nucleotide sugar transporter; UDP-glucuronic acid; UDP-N-acetylgalactosamine; endoplasmic reticulum; glucuronidation; chondroitin sulfate biosynthesis
Ask authors/readers for more resources
A novel human nucleotide sugar transporter (NST) which transports both UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) has been identified, cloned and characterized. The strategy for the identification of the novel NST involved a search of the expressed sequence tags database for genes related to the human UDP-galactose transporter-related isozyme 1, followed by heterologous expression of a candidate gene (hUGTrel7) in Saccharomyces cerevisiae and biochemical analyses. Significantly more UDP-GlcA and UDP-GalNAc were translocated from the reaction medium into the lumen of microsomes prepared from the hUGTrel7-expressing yeast cells than into the control microsomes from cells not expressing hUGTrel7. The possibility that this transporter participates in glucuronidation and/or chondroitin sulfate biosynthesis is discussed. (C) 2001 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available