Journal
BIOCHEMICAL SOCIETY TRANSACTIONS
Volume 42, Issue -, Pages 1211-1218Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BST20140105
Keywords
co-localization single-molecule spectroscopy (CoSMoS); dynamics; fluorescence resonance energy transfer (FRET); pre-mRNA splicing; single-molecule; fluorescence; splicessomal RNA
Categories
Funding
- National Institutes of Health [GM085116]
- CAREER award of the National Science Foundation [MCB-0747285]
- Clinical Sciences Center of the Medical Research Council
- Imperial College London
- MRC [MC_UP_1102/5] Funding Source: UKRI
- Medical Research Council [MC_UP_1102/5] Funding Source: researchfish
Ask authors/readers for more resources
Pre-mRNA (precursor mRNA) splicing is a key step in cellular gene expression where introns are excised and exons are ligated together to produce mature mRNA. This process is catalysed by the spliceosome, which consists of five snRNPs (small nuclear ribonucleoprotein particles) and numerous protein factors. Assembly of these snRNPs and associated proteins is a highly dynamic process, making it challenging to study the conformational rearrangements and spliceosome assembly kinetics in bulk studies. In the present review, we discuss recent studies utilizing techniques based on single-molecule detection that have helped overcome this challenge. These studies focus on the assembly dynamics and splicing kinetics in real-time, which help understanding of spliceosomal assembly and catalysis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available