Journal
VIROLOGY
Volume 283, Issue 1, Pages 78-83Publisher
ACADEMIC PRESS INC
DOI: 10.1006/viro.2001.0849
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Funding
- NHLBI NIH HHS [T23 HL07780-07] Funding Source: Medline
- NIAID NIH HHS [AI29306, T32 AI07492] Funding Source: Medline
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Human immunodeficiency virus type-1 (HIV-1) Vpr is a virion-associated protein implicated to have a role in AIDS pathogenesis. In regard to the amount of Vpr incorporated into virus particles, the published data vary widely. To address this, we quantitated Vpr in Virus particles derived from diverse sources that are used to evaluate the biological effect of Vpr. Virus particles from infected cells showed only a small amount of Vpr. Interestingly, virus particles from cells cotransfected with HIV-1 proviral DNA lacking Vpr coding sequences (NL Delta Vpr) and a Vpr expression plasmid showed a drastic increase (29.4-fold) in the incorporation of Vpr. Furthermore, cotransfection involving NL Delta Vpr and different concentrations of Vpr expression plasmid resulted in virus particles containing Vpr in proportion to the Vpr expression plasmid used. The differences in virus particles with respect to Vpr as revealed by these studies should be taken into account in assessing the effect of Vpr. (C) 2001 Academic Press.
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