Journal
LIFE SCIENCES
Volume 68, Issue 22-23, Pages 2481-2487Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0024-3205(01)01042-6
Keywords
muscarinic receptor; G protein; Ca2+ channel; M current
Funding
- NIDA NIH HHS [DA11322] Funding Source: Medline
- NINDS NIH HHS [NS08174, NS26920] Funding Source: Medline
Ask authors/readers for more resources
Many different G protein-coupled receptors modulate the activity of Ca2+ and K+ channels in a variety of neuronal types. There art: five known subtypes (M-1-M-5) Of muscarinic acetylcholine receptors. Knockout mice lacking the M-1, M-2, or M-4 subtypes are studied to determine which receptors mediate modulation of voltage-gated Ca2+ channels in mouse sympathetic neurons. In these cells, muscarinic agonists modulate N- and L-type Ca2+ channels and the M-type K+ channel through two distinct, G-protein mediated pathways. The fast and voltage-dependent pathway is lacking in the M-2 receptor knockout mice. The slow acid voltage-independent pathway is absent in the M-1 receptor knockout mice. Neither pathway is affected in the M-4 receptor knockout mice. Muscarinic modulation of the M current is absent in the M-1 receptor knockout mice, and can be reconstituted in a heterologous expression system using cloned channels and M-1 receptors. Our results using knockout mice are compared with pharmacological data in the rat. (C) 2001 Elsevier Science Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available