Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 283, Issue 1, Pages 124-129Publisher
ACADEMIC PRESS INC
DOI: 10.1006/bbrc.2001.4690
Keywords
-
Categories
Ask authors/readers for more resources
The efficiency of PAMAM dendrimer-mediated DNA transfer can be improved by the addition of substituted beta -cyclodextrins (beta -CDs) as formulation excipients. In vitro CAT expression increased approximately 200-fold when dendrimer/DNA/beta -CD formulations were applied on the surface of collagen membranes. The inclusion of beta -CD into the formulations resulted in particles that were smaller and more evenly distributed on the surface of the solid support. The average size of the complex formed at 50 mug/ml anal at charge ratio of 1 decreased from 156 nm to 5.8 nm and 21.2 nm in 0.025-0.1% w/vol beta -CDs. Sulfonated beta -CDs bind to dendrimer and in the increased concentration may displace DNA in the dendrimer/DNA complex. High concentrations of amphoteric beta -CD do not dissociate dendrimer/DNA complexes; however, they may decrease their ability to transfect cells. At the optimized formulations the surface-modified beta -CDs may enhance solid support-based transfection in vitro, through modification of dendrimer/DNA complex composition and improved surface distribution. (C) 2001 Academic Press.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available