Journal
SCIENCE
Volume 292, Issue 5517, Pages 733-737Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1058498
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Funding
- NIGMS NIH HHS [R01 GM010840-42, R01 GM010840-43, GM-32543, R01 GM010840, GM-10840] Funding Source: Medline
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Here we use mechanical force to induce the unfolding and refolding of single RNA molecules: a simple RNA hairpin, a molecule containing a three-helix junction, and the P5abc domain of the Tetrahymena thermophila ribozyme. All three molecules (P5abc only in the absence of Mg2+) can be mechanically unfolded at equilibrium, and when kept at constant force within a critical force range. are bi-stable and hop between folded and-unfolded states. We determine the force-dependent equilibrium constants for folding/unfolding these single RNA molecules and the positions of their transition states along the reaction coordinate.
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