Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 283, Issue 1, Pages 229-235Publisher
ACADEMIC PRESS INC
DOI: 10.1006/bbrc.2001.4713
Keywords
collagen; chromium (III) complexes; atomic force microscope; D-periodicity; conformation; aggregation
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Rat tail tendon (RTT) collagen has been reacted with a homologous series of chromium(III) complexes viz., (H2O)(4)Cr(OH)(2)Cr(H2O)(4)(4+) 1 (dimer), Cr-3(OH)(4)(H2O)(9)(5+) 2 (trimer), and Cr-4(OH)(4)(O)(2)(H2O)(12)(4+) 3 (tetramer), and the structural alterations brought about by these complexes have been investigated through atomic force microscopy (AFM) and circular dichroism (CD) studies. Examination of Cr(III)-treated tendons using AFM revealed changes in the D-periodicity of collagen, which may arise due to differences in the topological distribution of various Cr(III) complexes. Evidence for organisation of monomeric collagen into quarter staggered fibrils in the presence of Cr(III) dimer, 1, has been obtained. The quaternary structural changes induced by chromium in the protein have been correlated to the conformational changes of collagen in the absence of denaturation. (C) 2001 Academic Press.
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