Journal
JOURNAL OF CELL BIOLOGY
Volume 153, Issue 3, Pages 517-527Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.153.3.517
Keywords
aneuploidy; kinetochores; mitosis; microtubules; mitotic spindle checkpoint
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Funding
- NCRR NIH HHS [RR012019] Funding Source: Medline
- NIGMS NIH HHS [R01 GM059363-02, R01 GM059363, GM59363] Funding Source: Medline
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In mitotic cells, an error in chromosome segregation occurs when a chromosome is left near the spindle equator after anaphase onset (lagging chromosome). In PtK1 cells, we found 1.16% of untreated anaphase cells exhibiting lagging chromosomes at the spindle equator, and this percentage was enhanced to 17.55% after a mitotic block with 2 muM nocodazole. A lagging chromosome seen during anaphase in control or nocodazole-treated cells was found by confocal immunofluorescence microscopy to be a single chromatid with its kinetochore attached to kinetochore microtubule bundles extending toward opposite poles. This merotelic orientation was verified by electron microscopy, The single kinetochores of lagging chromosomes in anaphase were stretched laterally (1.2-5.6-fold) in the directions of their kinetochore microtubules, indicating that they were not able to achieve anaphase poleward movement because of pulling forces toward opposite poles. They also had inactivated mitotic spindle checkpoint activities since they did not label with either Mad2 or 3F3/2 antibodies. Thus, for mammalian cultured cells, kinetochore merotelic orientation is a major mechanism of aneuploidy not detected by the mitotic spindle checkpoint. The expanded and curved crescent morphology exhibited by kinetochores during nocodazole treatment may promote the high incidence of kinetochore merotelic orientation that occurs after nocodazole washout.
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