Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 52, Issue 358, Pages 901-910Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jexbot/52.358.901
Keywords
grapevine; Botrytis; stilbene synthase; inducible promoter; 'PR' proteins
Categories
Ask authors/readers for more resources
Resveratrol is a major phytoalexin in grapevine but its synthesis in response to phytopathogen attack decreases with grape berry ripening, A chimeric gene combining an alfalfa PR 10 promoter and Vst1 (Vitis stilbene synthase 1) gene was introduced into the genome of 41B rootstock, Transgenic plants were analysed for resveratrol production in leaves infected with Botrytis using an in vitro test. Among the 50 transgenic lines analysed, some exhibited a production lower than the non-transgenic control, but others accumulated resveratrol from 5-100-fold, Moreover, in the latter clones, symptoms were highly reduced in response to infection, These results were a good indication that the combination of a pathogen-inducible promoter and a defence gene may increase tolerance against fungi in grapevine, The efficacy of this approach should be further tested by experiments conducted in the vineyard.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available