Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 53, Issue 5, Pages 735-741Publisher
ROYAL PHARMACEUTICAL SOC GREAT BRITAIN
DOI: 10.1211/0022357011775866
Keywords
-
Categories
Ask authors/readers for more resources
We have developed an in-vitro model of monocyte-derived macrophage (MDMO) to compare fluoroquinolone uptake in monocytes and derived macrophages. Monocyte-derived macrophages were obtained in-vitro by cultivating freshly isolated monocytes for seven days in RPMI 1640 medium, containing foetal calf serum and Rhu granulocyte-macrophage colony stimulating factor. Final suspensions contained 95% viable cells and 63 % macrophages. Intramacrophagic accumulation of ciprofloxacin, ofloxacin or sparfloxacin was measured at equilibrium after 30-min incubation in the presence of 16-18 mug mL(-1) antibiotic. The results revealed low intra-cellular accumulation of ofloxacin in MDMO (intracellular/extracellular ratio: IC/EC = 1.7), Ciprofloxacin and sparfloxacin uptake was significantly higher. The IC/EC ratios were only slightly increased in macrophages when compared with monocytes under the same experimental conditions. These results suggest that maturation of monocyte to macrophage has only a limited effect on basal quinolone uptake. Monocytic maturation cannot explain the important differences between fluoroquinolone accumulation in monocytes and tissue macrophages. Cell activation may be a greater determinant.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available