Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 21, Issue 9, Pages 3083-3095Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.21.9.3083-3095.2001
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Funding
- NHLBI NIH HHS [HL20899, R01 HL020899] Funding Source: Medline
- NIDDK NIH HHS [R37 DK045365, R01 DK045365, R56 DK045365, DK45365] Funding Source: Medline
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The duplicated CCAAT box is required for gamma gene expression. We report here that the transcriptional factor NF-Y is recruited to the duplicated CCAAT box in vivo. A mutation of the duplicated CCAAT box that severely disrupts the NF-Y binding also reduces the accessibility level of the gamma gene promoter, affects the assembly of basal transcriptional machinery, and increases the recruitment of GATA-1 to the locus central region (LCR) and the proximal promoter and the recruitment of transcription cofactor CBP/p300 to the LCR. These findings suggest that recruitment of NF-Y to the duplicated CCAAT box plays a role in the chromatin opening of the gamma gene promoter as well as in the communication between the gamma gene promoter and the LCR.
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