Journal
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Volume 20, Issue 5, Pages 895-903Publisher
SPRINGER
DOI: 10.1007/s00775-015-1274-2
Keywords
DNA; Metal-mediated base pair; Silver; Nucleic acid
Funding
- Deutsche Forschungsgemeinschaft [SFB 858]
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A new artificial nucleoside 2 comprising 4-(2'-pyridyl)imidazole as the nucleobase was devised and incorporated into a series of DNA oligonucleotide duplexes. The propensity of 2 to form metal-mediated homo and hetero base pairs was investigated in various 13mer and 26mer duplexes. The artificial nucleoside readily forms Ag(I)-mediated homo base pairs of the type 2-Ag(I)-2, in which the Ag(I) ion resides in a [2 + 2] coordination environment. In addition, the combination with the previously established imidazole nucleoside 1 leads to the formation of Ag(I)-mediated hetero base pairs (2-Ag(I)-1 and 1-Ag(I)-2) with a [2 + 1] coordination environment. With an increase in the melting temperature of Delta T (m) = 21.8 A degrees C upon the formation of a Ag(I)-mediated base pair, the 2-Ag(I)-1 pair with the 13mer duplex is the most stabilizing Ag(I)-mediated base pair so far. Generally, the impact of the formation of a metal-mediated base pair on the properties of the DNA duplex was found to be significantly more prominent for shorter, intrinsically less stable duplexes.
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