Journal
THEORETICAL AND APPLIED GENETICS
Volume 102, Issue 6-7, Pages 1082-1087Publisher
SPRINGER-VERLAG
DOI: 10.1007/s001220000515
Keywords
kingdom and biological evolution; phylogenetic tree; RAPD-PCR; retrotransposon-like sequence; SCAR
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When developing SCARs by sequencing RAPD markers useful for olive variety identification, one RAPD sequence of 407 bp has been identified that shows significant DNA homology with more than 160 retrotransposon-like sequences. A generally coherent phylogenetic tree has been constructed based on the homologous retrotransposon-like sequences, reflecting genetic distances in 35 species belonging to eight kingdoms. The implications of this finding in the development of SCAR markers are discussed. In addition, specific oligonucleotide primers have been developed to amplify the DNA region and have a practical application as an internal amplification control in SCAR-based multiplexed PCRs. To our knowledge, this is the first report of a retrotransposon-like element in olive.
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