Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 49, Issue 5, Pages 2142-2145Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf001356x
Keywords
fatty acids; fatty amides; feed; digesta; methylation procedures
Ask authors/readers for more resources
Two direct methylation procedures often used for the analysis of total fatty acids in biological samples were evaluated for their application to samples containing fatty amides, Methylation of 5 mg of oleamide (cis-9-oetadecenamide) in a one-step (methanolic HCl for 2 h at 70 degreesC) or a two-step (sodium methoxide for 10 min at 50 degreesC followed by methanolic HCl for 10 min at 80 degreesC) procedure gave 59 and 16% conversions of oleamide to oleic acid, respectively. Oleic acid recovery from oleamide was increased to 100% when the incubation in methanolic HCl was lengthened to 16 h and increased to 103% when the incubation in methoxide was modified to 24 h at 100 degreesC. However, conversion of oleamide to oleic acid in an animal feed sample was incomplete for the modified (24 h) two-step procedure but complete for the modified (16 h) one-step procedure. Unsaturated fatty amides in feed and digesta samples can be converted to fatty acid methyl esters by incubation in methanolic HCl if the time of exposure to the acid catalyst is extended from 2 to 16 h.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available