Journal
GENES CHROMOSOMES & CANCER
Volume 31, Issue 1, Pages 96-98Publisher
WILEY-LISS
DOI: 10.1002/gcc.1122
Keywords
-
Categories
Ask authors/readers for more resources
We report an apparent BRCA I homozygous knockout that, on further analysis. was found to be an artefact of the polymerase chain reaction. This finding has two important implications. First, it challenges results of a previous study that reported a homozygous knockout associated with the same BRCA I mutation. Second, our findings suggest that mispriming caused by mismatched primers at the site of single-nucleotide polymorphisms, leading to preferential amplification of one allele, may represent a significant proportion of instances of mutation-detection insensitivity. This may have major implications for the sensitivity of all polymerase chain reaction-based mutation-detection methods in clinical genetic testing laboratories. (C) 2001 Wiley-Liss, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available