Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 290, Issue 15, Pages 9542-9554Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M114.596098
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Funding
- Fonds de la Recherche Scientifique Medicale of Belgium
- Actions de Recherche Concertees
- Interuniversity Attraction Poles Programme, Belgian State, Belgian Science Policy [P7-14]
- Belgian Fonds pour la formation a la Recherche dans l'Industrie et l'Agriculture
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The ability of G protein-coupled receptors (GPCRs) to activate selective signaling pathways according to the conformation stabilized by bound ligands (signaling bias) is a challenging concept in the GPCR field. Signaling bias has been documented for several GPCRs, including chemokine receptors. However, most of these studies examined the global signaling bias between G protein- and arrestin-de pendent pathways, leaving unaddressed the potential bias between particular G protein subtypes. Here, we investigated the coupling selectivity of chemokine receptors CCR2, CCR5, and CCR7 in response to various ligands with G protein subtypes by using bioluminescence resonance energy transfer biosensors monitoring directly the activation of G proteins. We also compared data obtained with the G protein biosensors with those obtained with other functional readouts, such as beta-arrestin-2 recruitment, cAMP accumulation, and calcium mobilization assays. We showed that the binding of chemokines to CCR2, CCR5, and CCR7 activated the three G alpha(i) subtypes (G alpha(i1); G alpha(i2), and G alpha(i3)) and the two G alpha(o) isoforms (G alpha(oa) and G alpha(ob)) with potencies that generally correlate to their binding affinities. In addition, we showed that the binding of chemokines to CCR5 and CCR2 also activated Ga-12, but not Ga-13. For each receptor, we showed that the relative potency of various agonist chemokines was not identical in all assays, supporting the notion that signaling bias exists at chemokine receptors.
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