Journal
CELL DEATH AND DIFFERENTIATION
Volume 8, Issue 5, Pages 486-494Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.cdd.4400835
Keywords
apoptosis; Bcl-w; spermatogenesis; monoclonal antibody
Categories
Funding
- NCI NIH HHS [CA80188] Funding Source: Medline
Ask authors/readers for more resources
Anti-apoptotic members of the Bcl-2 family, such as Bcl-w, maintain cell viability by preventing the activation of the cell death effectors, the caspases, Gene targeting experiments in mice have demonstrated that Bcl-w is required for spermatogenesis and for survival of damaged epithelial cells in the gut. Bcl-w is, however, dispensable for physiological cell death in other tissues. Here we report on the analysis of Bcl-w protein expression using a panel of novel monoclonal antibodies. Bcl-w is found In a diverse range of tissues including colon, brain and testes, A survey of transformed cell lines and purified hematopoietic cells demonstrated that Bcl-w is expressed in cells of myeloid, lymphoid and epithelial origin. Subcellular fractionation and confocal laser scanning microscopy demonstrated that Bcl-w protein is associated with intracellular membranes. The implications of these results are discussed in the context of the phenotype of Bcl-w-null mice and recent data that suggest that Bcl-w may play a role in colon carcinogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available