Adventitious bud induction in Eucalyptus globulus Labill.

Adventitious buds and shoots of Eucalyptus globulus Labill. (Tasmanian Bluegum) have been regenerated from cotyledons and hypocotyls from mature embryos and seedlings. Adventitious buds were induced at high frequency with 0.05 muM thidiazuron in combination with 0.2 muM 2,4-dichlorophenoxyacetic acid or 5 muM alpha -naphthaleneacetic acid. Culture of explants in the dark inhibited bud induction, but up to 86% of cotyledons, longitudinally split just prior to culture, produced adventitious buds in the light. Development of buds into shoots occurred only at low frequency, after transfer to media containing N-6-benzylaminopurine.