Journal
BIOCHEMICAL PHARMACOLOGY
Volume 77, Issue 9, Pages 1513-1521Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bcp.2009.02.005
Keywords
5-Lipoxygenase; Leukotriene; Prostaglandin E2; Inflammation; Garcinol
Categories
Funding
- Deutsche Forschungsgemeinschaft
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Garcinol (camboginol) from the fruit rind of Guttiferae species shows anti-carcinogenic and and inflammatory properties, but the underlying molecular mechanisms are unclear. Here we show that garcinol potently interferes with 5-lipoxygenase (EC 7.13.11.34) and microsomal prostaglandin (PG)E-2 synthase (mPGES)-1 (EC 5.3.99.3), enzymes that play pivotal roles in inflammation and tumorigenesis. In cell-free assays, garcinol inhibited the activity of purified 5-lipoxygenase and blocked the mPGES-1-mediated conversion of PGH(2) to PCE2 with IC50 values of 0.1 and 0.3 mu M, respectively. Garcinol suppressed 5-lipoxygenase product formation also in intact human neutrophils and reduced PGE(2) formation in interleukin-1 beta-stimulated A549 human lung carcinoma cells as well as in human whole blood stimulated by lipopolysaccharide. Moreover, garcinol interfered with isolated cyclooxygenase (COX)-1 (EC 1.14.99.1, IC50 = 12 mu M) and with the formation of COX-1-derived 12(S)-hydroxy-5-cis-8,10-trans-heptadecatrienoic acid and thromboxane B-2 in human platelets. In contrast, neither Ca2+-ionophore (A23187)-induced arachidonic acid release in neutrophils nor COX-2 activity in A549 cells or whole blood, measured as formation of 6-keto PGF(1 alpha), or isolated human recombinant COX-2 were significantly affected by garcinol (<= 30 mu M). Together, the high potency of garcinol to selectively suppress PGE(2) synthesis and 5-lipoxygenase product formation provides a molecular basis for the anti-inflammatory and anti-carcinogenic effects of garcinol and rationalizes its therapeutic use. (C) 2009 Elsevier Inc. All rights reserved.
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