Journal
BIOCHEMICAL PHARMACOLOGY
Volume 77, Issue 11, Pages 1709-1716Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bcp.2009.02.024
Keywords
Methylglyoxal; Mitochondria; Peroxynitrite; Complex III; Alagebrium
Categories
Funding
- Canadian Institutes of Health Research [MOP-68938]
- Heart and Stroke Foundation of Saskatchewan
- GREAT program of CIHR/Heart and Stroke Foundation of Canada
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The effects of methylglyoxal (MG) on mitochondria with specific foci on peroxynitrite (ONOO-) production, manganese superoxide dismutase (MnSOD) activity, and mitochondrial functions in vascular smooth muscle A-10 cells were investigated. Mitochondrial MG content was significantly increased after A-10 cells were treated with exogenous MG, and so did advanced glycated endproducts (AGEs) formation, indicated by the appearance of N-epsilon-(carboxyethyl) lysine, in A-10 cells. The levels of mitochondrial reactive oxygen species (mtROS) and ONOO- were significantly increased by MG treatment. Application of ONOO- specific scavenger uric acid lowered the level of mtROS. MG significantly enhanced the production of mitochondrial superoxide (O-2(center dot-)) and nitric oxide (NO), which were inhibited by SOD mimic 4-hydroxy-tempo and mitochondrial nitric oxide synthase (mtNOS) specific inhibitor 7-nitroindazole, respectively. The activity of MnSOD was decreased by MG treatment. Furthermore, MG decreased respiratory complex III activity and ATP synthesis in mitochondria, indicating an impaired mitochondrial respiratory chain. AGEs cross-link breaker alagebrium reversed all aforementioned mitochondrial effects of MG. Our data demonstrated that mitochondrial function is under the control of MG. By inhibiting Complex III activity, MG induces mitochondrial oxidative stress and reduces ATP production. These discoveries will help unmask molecular mechanisms for various MG-induced mitochondrial dysfunction-related cellular disorders. (c) 2009 Elsevier Inc. All rights reserved.
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