Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 39, Issue 5, Pages 433-442Publisher
GAUTHIER-VILLARS/EDITIONS ELSEVIER
DOI: 10.1016/S0981-9428(01)01250-5
Keywords
hypersensitive reaction; in situ histochemistry; Nicotiana tabacum; oxidative burst; ozone; ROS; TMV
Categories
Ask authors/readers for more resources
The pattern and time course of reactive oxygen species (ROS) production were monitored histochemically in vivo in tobacco (Nicotiana tabacum L.) cv. Bel W3 and cv. Bel B leaves, comparing the hypersensitive (HR)-like reaction induced by the abiotic stressor ozone and the response to tobacco mosaic virus (TMV). The applied assays (nitro blue tetrazolium, NBT; 2',7'-dichlorofluorescin diacetate, DCFH-DA; 3,3'-diaminobenzidine, DAB) gave positive results, evidencing scattered ROS production in a spotted pattern. DCFH-DA and DAB predominantly evidenced localised, long-lasting hydrogen peroxide production in cells prone to die, producing very similar pictures in TMV infected leaves of both tobacco cultivars and in ozone-sensitive Eel W3. Nitro blue tetrazolium reaction pointed out that a transient oxidative burst involving superoxide anion is induced early by ozone also in the tolerant cv. Eel B, despite the low malondialdehyde level. (C) 2001 Editions scientifiques et medicales Elsevier SAS.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available