Journal
BIOCHEMICAL JOURNAL
Volume 450, Issue -, Pages 427-432Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20121713
Keywords
dimethylallyl:tRNA([Ser]Sec) transferase; N-6-isopentenyladenosine (i(6)A); isopentenyl tRNA transferase (IPT); isopentenylation; selenium; tRNA modification
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Funding
- Junta de Comunidades de Castilla-La Mancha (Spain)
- Deutsche Forschungsgemeinschaft [Schw914/2-1]
- Charite-Universitatsmedizin Berlin
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Selenoproteins are proteins carrying the rare amino acid Sec (selenocysteine). Full expression of selenoproteins requires modification of tRNA([Ser]Sec), including N-6-isopentenylation of base A(37). We show that Trit1 is a dimethylallyl:tRNA([Ser]Sec) transferase. Knockdown of Trit1 reduces expression of selenoproteins. Incubation of in vitro transcribed tRNA([Ser]Sec) with recombinant Trit1 transfers [C-14]dimethylallyl pyrophosphate to tRNA([Ser]Sec). 37A>G tRNA([Ser]Sec) is resistant to isopentenylation by Trit1.
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