Journal
BIOCHEMICAL JOURNAL
Volume 443, Issue -, Pages 355-359Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20120249
Keywords
129 mouse strain; caspase 1; caspase 11; inhibitor of apoptosis protein (IAP); lipopolysaccharide (LPS); sepsis
Categories
Funding
- National Institutes of Health [R01 CA142809]
- American Asthma Foundation
- [T32 HL007517]
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A recent study revealed that ES (embryonic stem) cell lines derived from the 129 murine strain carry an inactivating mutation within the caspase 11 gene (Casp4) locus [Kayagaki, Warming, Lamkanfi, Vande Walle, Louie, Dong, Newton, Qu, Liu, Heldens, Zhang, Lee, Roose-Girma and Dixit (2011) Nature 479, 117121]. Thus, if 129 ES cells are used to target genes closely linked to caspase 11, the resulting mice might also carry the caspase 11 deficiency as a passenger mutation. In the present study, we examined the genetic loci of mice targeted for the closely linked c-IAP (cellular inhibitor of apoptosis) genes, which were generated in 129 ES cells, and found that, despite extensive backcrossing into a C57BL/6 background, c-IAP1(-/-) animals are also deficient in caspase 11. Consequently, data obtained from these mice should be re-evaluated in this new context.
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