Journal
BIOCHEMICAL JOURNAL
Volume 446, Issue -, Pages 225-233Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20120583
Keywords
acidic stress; lactic acid; processing body (P-body); Saccharomyces cerevisiae; stress granule; suppressor of clathrin deficiency 6 (Scd6)
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Funding
- Skylark Food Science Institute
- Japanese Ministry of Education, Culture, Sports, Science and Technology [23580113]
- Grants-in-Aid for Scientific Research [23580113] Funding Source: KAKEN
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The stress response of eukaryotic cells often causes an attenuation of bulk translation activity and the accumulation of non-translating mRNAs into cytoplasmic mRNP (messenger ribonucleoprotein) granules termed cytoplasmic P-bodies (processing bodies) and SGs (stress granules). We examined effects of acidic stress on the formation of mRNP granules compared with other forms of stress such as glucose deprivation and a high Ca2+ level in Saccharomyces cerevisiae. Treatment with lactic acid clearly caused the formation of P-bodies, but not SGs, and also caused an attenuation of translation initiation, albeit to a lesser extent than glucose depletion. P-body formation was also induced by hydrochloric acid and sulfuric acid. However, lactic acid in SD (synthetic dextrose) medium with a pH greater than 3.0, propionic acid and acetic acid did not induce P-body formation. The results of the present study suggest that the assembly of yeast P-bodies can be induced by external conditions with a low pH and the threshold was around pH 2.5. The P-body formation upon acidic stress required Scd6 (suppressor of clathrin deficiency 6), a component of P-bodies, indicating that P-bodies induced by acidic stress have rules of assembly different from those induced by glucose deprivation or high Ca2+ levels.
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