Journal
NATURE MEDICINE
Volume 7, Issue 5, Pages 631-634Publisher
NATURE AMERICA INC
DOI: 10.1038/87979
Keywords
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Funding
- NIAID NIH HHS [AI34786] Funding Source: Medline
- NIGMS NIH HHS [GM56553] Funding Source: Medline
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Early steps of infection by HIV-1 involve entry of the viral core into cells, reverse transcription to form the linear viral DNA, and integration of that DNA into a chromosome of the host. The unintegrated DNA can also follow non-productive pathways, in which it is circularized by recombination between DNA long-terminal repeats (LTRs), circularized by ligation of the DNA ends or degraded. Here we report quantitative methods that monitor formation of reverse transcription products, two-LTR circles and integrated proviruses. The integration assay employs a novel quantitative form of Alu-PCR that should be generally applicable to studies of integrating viruses and gene transfer vectors.
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