4.5 Article

Reversible membrane reorganizations during photosynthesis in vivo: revealed by small-angle neutron scattering

Journal

BIOCHEMICAL JOURNAL
Volume 436, Issue -, Pages 225-230

Publisher

PORTLAND PRESS LTD
DOI: 10.1042/BJ20110180

Keywords

chloroplast thylakoid membrane; cyanobacterium; diatom; light-induced reorganization; membrane ultrastructure; small-angle neutron scattering (SANS)

Funding

  1. European Union [238017]
  2. National Office for Research and Technology [80345, NAP-VENEUS05]
  3. Bourse du Gouvernement Francais
  4. Neutron facilities (DANSCATT)

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In the present study, we determined characteristic repeat distances of the photosynthetic membranes in living cyanobacterial and eukaryotic algal cells, and in intact thylakoid membranes isolated from higher plants with time-resolved small-angle neutron scattering. This non-invasive technique reveals light-induced reversible reorganizations in the seconds-to-minutes time scale, which appear to be associated with functional changes in vivo.

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