Hydrophobic interaction chromatography of proteins

In this article. an overview of hydrophobic interaction chromatography (HIC) of proteins is given. After a brief description of protein hydrophobicity and hydrophobic interactions, we present the different proposed theories for the retention mechanism of proteins in HIC. Additionally, the main parameters to consider for the optimization of fractionation processes by HIC and the stationary phases available were described. Selected examples of protein fractionation by HIC are also presented. (C) 2001 Elsevier Science B.V. All rights reserved.