Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 98, Issue 10, Pages 5705-5710Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.091468498
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Neocentromeres (NCs) are fully functional centromeres that arise ectopically in noncentromeric regions lacking Lu-satellite DNA. Using telomere-associated chromosome truncation, we have produced a series of minichromosomes (MICs) from a mardel(10) marker chromosome containing a previously characterized human NC. These MiCs range in size from approximate to0.7 to 1.8 Mb and contain single-copy intact genomic DNA from the 10q25 region. Two of these NC-based MI-Cs (NC-MiCs) appear circular whereas one is linear. All demonstrate stability in both structure and mitotic transmission in the absence of drug selection. Presence of a functional NC is shown by binding a host of key centromere-associated proteins. These NC-MiCs provide direct evidence for mitotic segregation function of the NC DNA and represent examples of stable mammalian MiCs lacking centromeric repeats.
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