Arabidopsis thaliana GLX2-1 contains a dinuclear metal binding site, but is not a glyoxalase 2

In all effort to probe the structure and function of a predicted mitochondrial glyoxalase 2, GLX2-1 from Arabidopsis thaliana, GLX2-1 was cloned, overexpressed, purified and characterized using metal analyses, kinetics, and UV-visible, EPR, and H-1-NMR spectroscopies. The purified enzyme Was purple and contained substoichiometric amounts of iron and zinc; however, metal-binding Studies reveal that GLX2-1 call bind nearly two equivalents of either iron or zinc and that the most stable analogue of GLX2-1 is the iron-containing form. UV-visible spectra of the purified enzyme Suggest the presence of Fe(II) in the protein, but the Fe(II) call be oxidized over time or by the addition of metal ions to the protein. EPR spectra revealed the presence of all anti-ferromagnetically-coupled Fe(III)Fe(II) centre and the presence of a protein-bound high-spin Fe(III) centre, perhaps its part of a FeZn centre. No paramagnetically shifted peaks were observed in H-1-NMR spectra of the GLX2-1 analogues, suggesting low amounts of the paramagnetic, anti-ferromagnetically coupled centre. Steady-state kinetic studies with several thiolester substrates indicate that GLX2-1 is not a GLX2. In contrast with all of the other GLX2 proteins characterized, GLX2-1 contains all arginine ill place of one of the metal-binding histidine residues at position 246. In order to evaluate further whether Arg(246) binds metal, the R246L Mutant was prepared. The metal binding results are very similar to those of native GLX2-1 Suggesting that a different amino acid is recruited as a metal-binding ligand. These results demonstrate that Arabidopsis GLX2-1 is a novel member of (lie metallo-beta-lactamase superfamily.