Journal
SCIENCE
Volume 292, Issue 5520, Pages 1385-1388Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1060418
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- NIDDK NIH HHS [DK 54942] Funding Source: Medline
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Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.
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