Journal
BIOCHEMICAL JOURNAL
Volume 410, Issue -, Pages 485-493Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20071367
Keywords
beta-cell; energy status; eukaryotic initiation factor 2 alpha (eIF2 alpha); glucose; protein kinase RNA-activated (PKR)-like endoplasmic reticulum kinase (PERK); translation
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Funding
- Wellcome Trust Funding Source: Medline
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In the present study, we demonstrate that, in pancreatic cells, eIF2 alpha (eukaryotic initiation factor 2 alpha) phosphorylation in response to a decrease in glucose concentration is primarily mediated by the activation of PERK [PKR (protein kinase RNA activated)-like endoplasmic reticulum kinase]. We provide evidence that this increase in PERK activity is evoked by a decrease in the energy status of the cell via a potentially novel mechanism that is independent of IRE1 (inositol requiring enzyme 1) activation and the accumulation of unfolded nascent proteins within the endoplasmic reticulum. The inhibition of eIF2a phosphorylation in glucose-deprived cells by the overexpression of dominant-negative PERK or an N-terminal truncation mutant of GADD34 (growth-arrest and DNA-damage-inducible protein 34) leads to a 53 % increase in the rate of total protein synthesis. Polysome analysis revealed that this coincides with an increase in the amplitude but not the number of ribosomes per mRNA, indicating that eIF2 alpha dephosphorylation mobilizes hitherto untranslated mRNAs on to polysomes. In summary, we show that PERK is activated at low glucose concentrations in response to a decrease in energy status and that this plays an important role in glucose-regulated protein synthesis in pancreatic beta-cells.
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